Structure and properties of the extracellular inulinase of Kluyveromyces marxianus CBS 6556
نویسندگان
چکیده
منابع مشابه
Structure and properties of the extracellular inulinase of Kluyveromyces marxianus CBS 6556.
In the yeast Kluyveromyces marxianus two forms of inulinase were present, namely, an inulinase secreted into the culture fluid and an inulinase retained in the cell wall. Both forms were purified and analyzed by denaturing and nondenaturing polyacrylamide gel electrophoresis. With the use of endo-beta-N-acetyl-glucosaminidase H, it was established that the enzyme retained in the cell wall and t...
متن کاملContinuous-culture study of the regulation of glucose and fructose transport in Kluyveromyces marxianus CBS 6556.
Regulation of transport of D-glucose and D-fructose was studied in Kluyveromyces marxianus grown in continuous culture. Both substrates could be transported by at least two different transport systems, low-affinity transport and high-affinity proton-sugar symport. The low-affinity transporter, specific for both glucose and fructose, was constitutively present and was apparently not regulated by...
متن کاملProduction of inulinase from Kluyveromyces marxianus using dahlia tuber extract
Various carbon sources were evaluated for production of inulinase by yeast, Kluyveromyces marxianus MTCC 3995. Highest inulinase activity was observed with Dahlia extract (25.3 nkat mL(-1)) as carbon source. The enzyme activity was 1.4 folds higher than that observed in media containing pure chicory inulin (17.8 nkat mL(-1)). The yeast showed good growth on a simple medium containing dahlia ext...
متن کاملOPTIMIZATION OF INULINASE PRODUCTION USING COPRA WASTE BY Kluyveromyces marxianus var. marxianus
Kluyveromyces marxianus var. marxianus was found to secrete a large amount of extracellular inulinase in to the medium. The optimization of inulinase production using copra waste as a carbon source was performed with statistical methodology based on experimental designs. The screening of eighteen nutrients for their influence on inulinase production was achieved using a Plackett– –Burman design...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1990
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.56.11.3337-3345.1990